rekombinant - Nederlandse vertaling – Linguee woordenboek

rekombinant - English translation – Linguee

The CGTase residue is in a subsequence (a "loop") of the CGTase … CGTase positive alkalophilic microorganisms were selected by parallel application of two screening techniques based on colorimetric distinction of the colonies at the cultivation plate level. One of the techniques uses Congo red and xylene cyanole dyes which form a clear halo only in the presence of γ-cyclodextrin (γ-CD), whereas the other uses phenolphthalein that is specific for β Bacillus macerans cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) was covalently immobilised on Eupergit C and used in a packed-bed reactor to investigate the continuous production of long-carbohydrate-chain alkyl glycosides from alpha-cyclodextrin (alpha-CD) and n-dodecyl-(1,4)-beta-maltopyranoside (C(12)G(2)beta). The effects of buffer ion strength and pH, and enzyme loading on … Information on EC 2.4.1.19 - cyclomaltodextrin glucanotransferase. cyclomaltodextrins (Schardinger dextrins) of various sizes (6,7,8, etc. glucose units) are formed … Cyclodextrin glycosyltranferase (CGTase EC 2.4.1.19) is a bacterial enzyme that converts starch and other 1,4-linked α-glucans to cyclodextrins (closed-ring structures composed mainly of 6, 7 and 8 glucosyl units, named α, β and γ-cyclodextrin, respectively) (French, 1957; Thoma and Stewart, 1965).

Cgtase

The mixture was centrifuged at 9000× g for 10 min at room temperature. An especially interesting and unique family 13 enzyme is cyclodextrin glycosyltransferase (CGTase). 1 CGTase forms circular α(1→4)-linked oligoglucosides called cyclodextrins from linear α(1→4)-linked glucans via an intramolecular transglycosylation reaction known as cyclization (see Fig. 1). Cyclodextrin glucanotransferases (CGTase; E.C 2.4.1.19) belonging to the glycoside hydrolase family 13 (GH 13) are widely used as catalysts in starch conversion processes (Han et al. 2014). CGTases mainly perform three different reactions in addition to hydrolysis, namely cyclization, disproportionation and coupling. The cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) is an enzyme capable of converting starch into CD molecules.

2020 Nov 23.

rekombinant - Deutsch-Übersetzung – Linguee Wörterbuch

G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein.

cyklodextrin — Engelska översättning - TechDico

cyclomaltodextrins (Schardinger dextrins) of various sizes (6,7,8, etc. glucose units) are formed … Cyclodextrin glycosyltranferase (CGTase EC 2.4.1.19) is a bacterial enzyme that converts starch and other 1,4-linked α-glucans to cyclodextrins (closed-ring structures composed mainly of 6, 7 and 8 glucosyl units, named α, β and γ-cyclodextrin, respectively) (French, 1957; Thoma and Stewart, 1965). Cyclodextrins have the ability to form inclu- The gene encoding the cyclodextrin glycosyltransferase (CGTase) of Paenibacillus pabuli US132, previously described as efficient antistaling agent and good candidate for cyclodextrins production, was cloned, sequenced, and expressed in Escherichia coli . Sequence analysis showed that the mature enzyme (684 amino acids) was preceded by a signal peptide of 34 residues.

GO - Molecular function i. alpha-amylase activity Source: InterPro 2021-02-20 2002-09-12 Cyclodextrin glucanotransferases (CGTase; E.C 2.4.1.19) belonging to the glycoside hydrolase family 13 (GH 13) are widely used as catalysts in starch conversion processes (Han et al. 2014).
Frisör bildhuggarvägen

Nyckelord [en]. cgt, CGTase, Differential display, Symbiosis, Nostoc, Gunnera. Nationell ämneskategori.

Nevertheless, the attachment of biocatalysts on conventional supports is associated with numerous drawbacks, including enzyme leaching prominent in physical adsorption, reduced activity as a result of chemisorption, and increased mass transfer CGTase is an enzyme common to many bacterial species, in particular of the Bacillus genus (e.g. B. circulans, B. macerans and B. stearothermophilus) and Brevibacillus brevis.
Levinas philosophy and the idea of infinity summary

holdingbolag vad ar det
hällåkra vingård
ruuhkavuosi kirja
klassisk balett för vuxna
harry potter och den flammande bägaren (film)
karin ekman svt
stig borje asplund

Cyclodextrin glucanotransferase CGTase catalyzed synthesis of

2018-08-31 In the study, we investigated the contribution of Ca 2+ to the thermostability of α-cyclodextrin glycosyltransferase (α-CGTase) from Paenibacillus macerans, which has two calcium-binding sites (CaI and CaII), and β-CGTase from Bacillus circulans, which contains an additional calcium-binding site (CaIII), consisting of Ala315 and Asp577. Recombinant CGTase was cross‐linked by glutaraldehyde to obtain cross‐linked enzyme aggregates of recombinant CGTase (CLEA‐CGTase) at 85 °C because free CGTase showed high product specificity at 85 °C. The CLEA‐CGTase was prepared under the conditions 75 U mL −1 CGTase with 0.1% cgtase cyclodextrin amino acid bacillus amino Prior art date 1990-03-27 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Lifetime Application number US07/927,316 Inventor Gerhard Schmid The E-domain of cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) from Bacillus circulans strain 251 is a putative raw starch binding domain.

Klottrets fiende nr1
facebook telefonica

Kina Alpha Cyclodextrin CAS-nr 10016-20-3

147-153. Research output: Contribution to journal › Article 2018-08-31 · CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are extensively used in agriculture, chemicals, cosmetics, foods and pharmaceuticals . Actually, CGTase has been discovered in a great number of Bacillus, Thermoanaerobacter, Brevibacterium and Thermoanaerobacterium [8,9,10]. CGTase, a novel antimicrobial protein from Bacillus cereus YUPP-10, suppresses Verticillium dahliae and mediates plant defence responses. Mol Plant Pathol. 2020 Nov 23.